Characterization of Bioactive compound isolated from Myrothecium spp. with UV, FTIR and HPLC Analysis

Abstract

Development of new drugs, especially in area of infectious diseases, represents today one of the most
important research. Fungal isolates are receiving increasing attention by natural product chemists due to
their diverse and structurally unprecedented compounds making them interesting candidates for drug
discovery. In fact, need for novel, safe and more efficient antibiotics is a key challenge to the
pharmaceutical industry today, moreover, increase in opportunistic infections in the immune
compromised host has influenced this demand. Nowadays, evaluating morphological and biochemical
differences as well as studying fungal genetic diversity via molecular indicators seem to be the most
common method for screening this genus. In this research we evaluate the potential of bioactive
compound, production and characterize the UV and FTIR spectroscopy and HPLC (High performance
liquid chromatography) analysis pattern of isolated Myrothecium spp. MRP001. Process development
for high level production of bioactive compound was applied using OFAT method. Then, following the
extraction of secondary metabolite, the UV and FTIR spectroscopy analysis was carried out for
characterization of the various extracts. Considering the coordinate analysis of UV and FTIR
spectroscopy pattern, the isolate MRP001 with substantial antimicrobial activity exhibited absorption at
3411 cm-1 which is indicator of hydroxyl groups, absorption at 2856 and 2915 cm-1 indicating
hydrocarbon chassis, and absorption at 1649 cm-1 indicating a double bond of polygenic compound.
These results highlight the importance of Myrothecium isolates in antibiotic production. HPLC
confirmed the production when compared with standards