Bacteriostatic activity of con a lectin from Canavalia ensiformis

S.R. Kulkarni, V. J. Tayade

Abstract


The aim of this work was to explore the therapeutic applications of Con A lectin from
Canavalia ensiformis and to explore its antibacterial activity. Activity of lectin was quantified
by their ability to agglutinate erythrocytes using Hemagglutination assay. Characterization
and purity of Con A lectin was evaluated by using SDS-PAGE analysis. The reversal of
hemagglutination activity of lectin was evaluated by using the sugars namely; mannose,
galactose, lactose, fructose, glucose. The antibacterial activity of lectins was tested against
Streptococcus mutans, Staphylococcus aureus, Bacillus subtilis, Escherichia coli using pour
plate method. Amoxycillin was used as standard. At 250mg/ml concentration Con A lectin
showed good bacteriostatic activity.

Keywords


Con A, Gel Electrophoresis, Hemagglutination Assay.

Full Text:

Full text


DOI: https://doi.org/10.30750/ijpbr.1.4.11

Refbacks

  • There are currently no refbacks.