Development and Validation of Dual Wavelength UV Spectrophotometric Method for simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in Tablet dosage form

Isha J Soni, Isha J Soni

Abstract


A simple and economical dual wavelength spectrophotometric method has been developed for
the simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in their tablet dosage
form. The principle for dual wavelength method is “the absorbance difference between two
points on the overlain spectra is directly proportional to the concentration of the component of
interest”. From the UV absorption spectrum of Cilnidipine, three wavelengths were selected,
which were 282.99, 337.85 and 352.92 nm. At 352.92 nm only Cilnidipine has reasonable
absorbance, so it was selected for the estimation of it from combination drug product. At
these two wavelengths absorbance for Cilnidipine was found to be same i.e. absorbance
difference was zero for any concentration, while for Olmesartan Medoxomil concomitantly
increase in absorbance difference with increase in its concentration. So, 282.99 and 337.85
nm wavelengths were selected for the estimation of Olmesartan Medoxomil from its
combination drug product. The method involved solving of an equation based on
measurement of absorbances at two wavelengths 282.99 and 337.85 nm. Regression analysis
of Beer’s plots showed good correlation in concentration range of 10-60 µg/ml for Cilnidipine
and 20-120 µg/ml for Olmesartan Medoxomil. The suitability of this method was for
quantitative determination of Cilnidipine and Olmesartan Medoxomil was proved by
validation and recovery study. The proposed method was found to be simple, rapid,
economical, accurate and reproducible for the routine analysis of both drugs in tablet dosage
forms.

Keywords


Cilnidipine ,Dual wavelength method ,Olmesartan Medoxomil,UV spectrophoto- metric method

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DOI: https://doi.org/10.30750/ijpbr.2.1.12

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