Immunoformatics and Molecular Simulation Study Unfold the Immunological Repertoire of Sabia Virus Glycoprotein G1

Abstract

Sabia virus Glycoprotein G1 (GP1) is a peripheral membrane protein involved in viral adsorption and consequential pathogenic infection. Glycoprotein G1 (GP1) was subjected to immunoformatic and molecular simulation study to predict effective B-cell and T-cell epitopes with knowledge-based exploration of probable immunological responses the epitopes may elicit. The linear and conformational epitopes of GP1 for B-cell were predicted by immunoformatic tools housed in IEDB recourse. Both CD4+ and CD8+ T-cell epitopes were predicted exploiting IEDB recourse tools whereas CD8+ T cell epitopes were severed further by immune response evoking ability of epitope-MHC complex. Contribution of GP1 to innate antiviral response was evolved by interferon (IFN)-gamma inducing and imitation capacity by immunoformatic and docking study respectively. The results of the B-cell epitope analysis suggest the occurrence of potential linear and conformational epitope with cross-reactivity. A range of T-cell epitopes were assumed to be involved in MHC class I and MHC class II molecule dependent antigen presentation. Glycoprotein G1 (GP1) may induce the IFNgamma but its IFN-gamma mimicking ability (confirmed by docking study) that adduces a prompt immune defense. Therefore, inherent immunological repertoire of GP1 epitopes can be taken in to advantage in future immunization regiment development against Sabia virus.