Single-Run method for Antibiotics in Honey through UPLC-MS/MS

Abstract

The current study focused on the development of a single method for quantitative estimation of the compounds of honey and for the same it is essential to document the compounds present in the honey found in several regions of India. Tylosine tartarate, erythromycin adihydrate, 4-epichlor otetracycline, enrofloxacin, sulfaqui noxaline, sulfachl oropyridazine are a few among the compounds predominate in honey. Chromatography-tandem mass spectrometry (UPLC-MS/MS) is being used to determine the compounds 3-amino-2-ozaxolidinone (AOZ, the marker residue for furazolidone), 3-amino-5- morpholino-methyl-1,3-oxa-zolidinone (AMOZ, the marker residue for furaltadone), 1-aminohydantoin (AHD, the marker residue for nitrofurantoin), and semicarbazide (SEM, the marker residue for nitrofurazone) present in the honey in one single analysis.