Alleviation of Sodium Arsenite Driven Antioxidant Status and Hepatotoxicity by N-Acetyl Cysteine (NAC)

Abstract

Arsenic has gained popularity for causing multi-organ health ailments. The probable hazardous influence of arsenic stands upon oxidative stress burden and accelerated free radical creation. The usual recovery process against arsenic toxicity has countless opposing consequences. Hence, this experiment was considered to assess the effect of NAC in arsenic imposed antioxidant status and hepatotoxicity. A total of 24 Wistar rats were arbitrarily assigned to four individual groups and the treatment protocol was sustained for 8 successive days. Here, sodium arsenite and NAC was introduced at the dose of 10mg/kg body weight and 100mg/kg body weight respectively. The hepato-renal antioxidant status, serum SGOT, SGPT, urea and creatinine level, status of pro-inflammatory cytokines and hepatic architecture were determined. Sodium arsenite exposed group manifested a remarkably high level of oxidative stress which was further evidenced by diminished antioxidant enzyme levels in hepato-renal tissues. Altered and upgraded level of SGOT and SGPT in arsenic challenged group were a hint of hepatic damage. Along with this, the disarrangement of the cellular structure noticed in hepatic architecture in arsenic exposed group supported hepatoxicity. Moreover, an advanced level of pro-inflammatory cytokines and reduced B-vitamins in the circulation confirmed the toxicity caused by arsenic. NAC application reserved the antioxidant status, improved B-vitamins, and additionally lessened the level of inflammation-causing cytokines and improved the regular histoarchitecture of liver in arsenic ingested animals, suggesting its antioxidant, anti-inflammatory and hepato-protective benefits of NAC.